This paper presents an application of images segmentation in HSI (Hue, Saturation, and Intensity) colour space for evaluation of one sensory characteristic of white brined cheese quality- cheese structure. It is developed a Java application for multi-level images segmentation and the results are compared with experts’ evaluation of cheese structure. A correlation analysis shows that a combination of two colours in segmented image with four colours, could be used for structure evaluation of white brined cheese.